Determination of translocations in neoplastic diseases
PCR for B lymphoid neoplasms
(9; 22) (q34; q11.2); BCR-ABL1 p190 t (9; 22) (q34; q11.2) BCR-ABL p210
t (4; 11) (q21; q23) MLL-AF4
t (12; 21) (p13; q22) TEL-AML1
(1; 19) (q23; p13.3) EA2-PBX1
(X; 11) (q13; q23) MLL; FOX04
(6; 11) (q27; q23) MLL; MLLT4
t (11,19) (q23; p13.1) MLL; ELL
t (10,11) (p12, q23) MLL; MMLT10
t (1,19) (p32; q23) MLL; EPS15
t (11,17) (q23; q12-21) MLL; MLLT6
(11; 19) (q23; p13.3) MLL; MLLT1
t (9; 11) (p22; q23) MLL; MLLT3
t (1,19) (q23; p13) TCF; PBX1 (PRL)
(17,19) (q22; p13) TCF; HLF
(12; 21) (p13; q22) ETV6; RUNX1
TAL1 (40 Kb Del) STILL; TAL1
t (4,11) (q21; q23) MLL; AFF1
t (1,11) (q21; q23) MLL; MLLT11
PCR for acute myeloid neoplasms
t (8,21) (q22; q22) RUNX1; RUNX1T1
t (3,21) (q26; q22) RUNX1; MSD1
t (16; 21) (p11; q22) FUS; ERG
t (15,17) (q22; q12) PML; STRANGE
inv. (16) (p13; q22) CBFB; MYH11
t (9; 22) (q34; q11) BCR; ABL
t (9,12) (q34; p13) ETV6; ABL1
t (5,12) (q33, p13) ETV; PDGFRB
t (12; 22) (p13; q11-12) ETV6; MN1
t (6,9) (p23; q34) DEK; NUP214
t (9,9) (q34; q34) SET; NUP214
t (3; 21) (q26; q22) EAP; EV11
11; 17) (q23; q12-21) ZBTB16; STRANGE
t (3,5) (q25.1, q35) NPM1; MLF1
t (9,22) (q34; q11) BCR; ABL e1a2
Determination of the Philadelphia chromosome t (9; 22) using the FISH technique
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Karyotype analysis from bone marrow or peripheral blood samples
Immunophenotype analysis for the determination of leukemias through flow cytometry
Panel 1:
CD8, CD4, CD19, CD33, CD10, CD34, CD5, CD20, CD3, CD45
Panel 2:
CD15, CD66c, CD19, CD56, CD123, CD34, CD28, CD9, CD81, CD45
Panel 3:
TdT, CD79, CD19, MPO, CD10, CD22, CD3
